| Abstract Detail
Systematics Section / ASPT Beckmann, Kevin G. [1], Wall, P. Kerr [2], Ma, Hong [2], Leebens-Mack, Jim [2], dePamphilis, Claude W. [3]. Identifying Single-copy Genes in Arabidopsis, Oryza, and Populus and Testing Their Utility for Plant Molecular Systematics Their Utility for Plant Molecular Systematics. Recent studies have indicated that ancient polyploidy is common across angiosperm lineages and in fact the genomes of all angiosperms may have been influenced by at least one genome-wide duplication event. Despite such events, single-copy, apparently orthologous gene sets have been identified in a broad range of angiosperms. Selection against duplicates may be maintaining these genes as single copy. If these genes have been maintained as single copy through angiosperm history, they should be quite useful for comparative genetic and physical mapping, and phylogenetic analyses. TribeMCL was used to cluster all genes in the Arabidopsis, Oryza, and Populus proteomes. Single-copy gene families were then identified from the large set of resulting clusters. Putative orthologs in other species were identified in searches of public databases (GenBank, TIGR Gene Index, and Plant Genome Network). These data sets are analyzed for their phylogenetic utility for molecular systematics.
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1 - Pennsylvanian State University, Department of Biology, 403 Life Sciences Building, University Park, Pennsylvania, 16802, USA
2 - Pennsylvanian State University, Department of Biology, University Park, Pennsylvania, 16802, USA
3 - Pennsylvania State University, Department of Biology and Institute of Molecular Evolutionary Genetics, University Park, Pennsylvania, 16801, USA
Keywords:
molecular marker
Phylogenetics
single-copy
orthologs
mapping
polyploidy
maintenance.
Presentation Type: Oral Paper
Session: 38-12
Location: 400/Hilton
Date: Tuesday, August 16th, 2005
Time: 5:00 PM
Abstract ID:313 |
