| Abstract Detail
Recent Topics Posters Hammer, Rick L. [1], Johnston, Spencer [2], Price, H. James [3], Hodnett, George [4], Manhart, James [1], Hatch, Stephan [6]. Flow cytometric measurement of nuclear DNA content in the Dichanthelium acuminatum complex (Poaceae). Flow cytometry was used to measure the nuclear DNA content of four of the subspecies in the D. acuminatum grass complex, D. a. acuminatum, D. a. leucothrix, D. a. lindheimeri and D. a. longiligulatum. Also analyzed was D. wrightianum which in the past has been included in the D. acuminatum complex. Nuclei were isolated from leaf tissue of each of the taxa, stained with propidium iodide, and the relative fluorescence intensity measured. Sorghum bicolor Tx623 (2C DNA content = 1.67 pg) was used as an internal standard and the DNA amount determined as the ratio of the sample to the standard times the DNA amount in the standard. The 2C DNA contents ranged from 1.335 pg in D. acuminatum subsp. longiligulatum to 1.687 pg in D. acuminatum subsp. lindheimeri. All 2C mean comparisons, except those of D. acuminatum subsp. acuminatum and subsp. leucothrix were significantly different. Interestingly, both subsp. acuminatum and subsp. leucothrix are morphologically similar. The D. acuminatum complex is a taxonomically difficult group with much overlap of morphological characters. Data from the present study will be combined with data from a molecular phylogenetic study and a phenetic analysis of morphological characters to evaluate the current subspecific taxonomic alignments within the species complex.
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1 - Texas A&M University, Department of Biology, Mail Stop TAMUS 3258, College Station, Texas, 77843, USA
2 - Texas A&M University, Department of Entomology, College Station, Texas, 77843, USA
3 - Texas A&M University, Department of Soil and Crop Sciences, Mail Stop 2474, College Station, Texas, 77843-2474, USA
4 - Texas A&M University, Department of Soil and Crop Sciences, College Station, Texas, 77843, USA
5 - Texas A&M University, Department of Biology, Mail Stop TAMUS 3258, College Station, Texas, 77843, USA
6 - Texas A&M University, Department of Rangeland Ecology and Management, Mail Stop TAMUS 2126, College Station, Texas, 77843, USA
Keywords:
Poaceae
Dichanthelium acuminatum
DNA content
genome size
flow cytometry.
Presentation Type: Recent Topics Poster
Session: 33-135
Location: Salon C, D & E - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 12:30 PM
Abstract ID:650 |
